Titus Thorne

Last Updated March 13, 2023

Titus Thorne

 March 13, 2023

Curious about Bacteriostactic Water for HGH?

Scientific researchers know that lab supplies play an important role and that not all tools are created equally. This is especially true when preparing therapeutic compounds such as hormones and peptides for injection.

Human Growth Hormone (HGH), for example, is widely studied and applied in therapeutic contexts. Administered via injection, it requires a sterile solvent to enable aqueous delivery. Bacteriostatic water is the preferred solvent for reconstituting and injecting HGH.

In this comprehensive guide, we will discuss why bacteriostatic water is the best complement to HGH for injection. This will include the features and benefits of both HGH and bacteriostatic water.

Read on to learn how to pair bacteriostatic water and HGH.

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What is Bacteriostatic Water?

Formulated as a sterile solvent for injections, bacteriostatic water consists of sterile water and an organically derived preservative agent (0.9% benzyl alcohol). The bacteriostat prevents the growth of bacteria to reduce the risk of contamination [1].

Bacteriostatic water is suitable for parenteral injections via subcutaneous, intravenous, and intramuscular routes [1, 2]. It has an average pH level of 5.7 and is available to qualified researchers and laboratory professionals [3].

The key benefit of bacteriostatic water is that it remains sterile for up to four weeks after the first dose is drawn. Sealed vials remain sterile for up to several years [4]. Its minimal interference with most therapeutic compounds, as well as its low toxicity, make bacteriostatic water an ideal solvent for injections. However, it is not recommended for newborns and subjects on certain drugs [3, 5].

Bacteriostatic Water for HGH

What is Bacteriostatic Water Used For?

  1. Bacteriostatic water is used as a solvent to reconstitute lyophilized powders before injection.
  2. It is also used as a dilutant to achieve the proper concentration of liquids before
  3. Due to its 0.9% benzyl alcohol content, bacteriostatic water remains sterile for up to four weeks after opening, allowing multiple doses from a single vial [4].
  4. It provides good solubility and interferes minimally with most therapeutic substances [6].
  5. The solution may be used to reconstitute or dilute various medications, peptides, and hormones for injection [7].


This solvent is not to be used in spinal injections [8].

Experts warn that bacteriostatic water should never be injected alone without a solute. Injection of pure bacteriostatic water can lead to hemolysis and the destruction of red blood cells. Adverse reactions include blood clots, injection site infection, and fever. If these symptoms appear, discontinue use [9, 10].

What is HGH?

HGH (Human Growth Hormone), also known as somatropin or GH, is a hormone that is naturally produced and secreted by the pituitary gland.

It plays a central role in regulating physical development from birth to adulthood, stimulating the growth of bone and other tissues. In adulthood, HGH has ongoing importance in regulating metabolic functions. It is key to maintaining body composition, bone density, and cardiovascular health [11, 12, 13].

HGH deficiency occurs in both children and adults with varying causes and symptoms. In children, the cause is often genetic or unknown. Symptoms include small stature, younger appearance than peers, poor hair growth, late puberty, and excess body fat. Low levels of HGH typically continue throughout adulthood [14].

In adults, HGH deficiency is typically caused by pituitary damage or tumors. Other possible causes include head injury, radiation therapy, and infection. Low HGH levels present many unpleasant symptoms in adults, such as [14]:

  • Fatigue
  • Reduced cognitive function
  • Excess body fat on the waistline
  • Cardiovascular problems
  • Depression and anxiety
  • Weakness in the bones and muscles

If HGH deficiency is suspected, tests will be performed to establish a proper diagnosis. As one can see, HGH is indispensable to proper development and ongoing health into adulthood.

Bacteriostatic Water for HGH

What is HGH Used For?

Synthetic HGH has been in use for decades as an FDA-approved treatment to address symptoms of various conditions.

Approved uses of HGH injections include treatment of poor growth in children due to [15, 16]:

  • HGH deficiency
  • Renal failure
  • Genetic conditions such as Turner syndrome and Prader-Willi syndrome.

It is prescribed for adults to treat:

  • HGH deficiency
  • Short bowel syndrome
  • Muscular degeneration due to HIV/AIDS

The benefits of HGH therapy in adult subjects diagnosed with HGH deficiency include [17]:

  • Enhanced body composition (reduced body fat and increased lean muscle mass)
  • Improved physical performance and exercise capacity
  • Boost in energy levels
  • Reduced risk of cardiovascular disease

Its anabolic effects have made HGH the subject of ongoing studies for its potential application in a range of clinical contexts beyond the scope of hormone deficiency, such as [16]:

  • Improved wound and injury healing
  • Reproductive health
  • Anti-aging effects
  • Athletic performance
  • Treatment of symptoms of cardiovascular and inflammatory diseases

Bacteriostatic Water for HGH

Subcutaneous injection is considered the most effective manner of HGH delivery, as it provides optimal bioavailability and rate of diffusion [18].

Daily injection several hours before bedtime is recommended, as this complements the body’s physiological patterns of endogenous HGH release [19].

HGH is typically available in lyophilized powder form and requires reconstitution with a solvent before injection.

Most experts recommend bacteriostatic water as the best solvent for the reconstitution and injection of HGH. Here, we will explore why bacteriostatic water is the go-to choice for qualified researchers, as well as how to properly use bacteriostatic water for HGH.

Why is Bacteriostatic Water Best for HGH Injections?

  1. Bacteriostatic water is the best option for injections of reconstituted solutes. Its preservative agent reduces the risk of infection without compromising the integrity of most therapeutic substances.
  2. Considering the daily injection schedule of HGH, it is convenient to have reconstituted vials on hand. Bacteriostatic water solutions are uniquely suited to storage and use for up to several weeks. This eliminates the need to reconstitute hormones daily, which would be inconvenient and time-consuming.
  3. The extended shelf life of reconstituted HGH solutions made with bacteriostatic water allows handlers to adjust the concentration as needed without having to discard vials, saving both time and money.

How is Bacteriostatic Water Used with HGH?

The method of HGH reconstitution for injection is as follows [19, 20]

  1. Assemble all necessary supplies, including bacteriostatic water, HGH lyophilized powder, alcohol swabs, a sterile syringe, and sterile needles.
  2. Clean the vials with an alcohol wipe to prevent contamination.
  3. Using the syringe, withdraw about 1mL of bacteriostatic water.
  4. Insert the needle into the HGH vial and gradually release the solvent. Let the water stream down the walls of the vial for a smooth dissolution.
  5. Do not shake or stir the vial, but gently rotate it. Allow the HGH powder to fully dissolve.
  6. The solution will appear clear when it is fully mixed. Don’t use cloudy solutions.
  7. Reconstituted HGH solutions should be stored in the refrigerator.
  8. HGH injections are best delivered in the thigh, waist, or deltoid with the needle at a 30-45° angle. It is recommended to rotate the injection sites to prevent lipoatrophy.

Exact dosages, administration schedules, and storage practices are subject to variation. Always follow relevant instructions.

As you can see, bacteriostatic water is an ideal solvent for use in HGH injection therapies.

Bacteriostatic Water for HGH

Where to Buy Bacteriostatic Water Online | 2023 Guide

If you are interested in adding bacteriostatic water to your toolkit, we recommend that you visit:


The trusted online vendor sells not only top-quality bacteriostatic water but all the supplementary items that are required for safe and smooth injections when carrying out your research.

Rather than wasting effort and time trying to source your kit from multiple retailers, pick up one of the deluxe sets available from this reputable site.

The starter kit includes:

  • 200 Alcohol Prep Pads
  • 100 Insulin Syringes (0.5 cc/mL x 29g x ½)
  • 3 vials of Bacteriostatic Water (30mL)
  • 10 Large Needles + Syringes Combo (3cc x 21g x 1)
  • 1 Sterile Empty Glass Vial (10mL)

The premium kit has even more:

  • 200 Alcohol Prep Pads
  • 200 Insulin Syringes (0.5 cc/mL x 29g x ½)
  • 5 vials of Bacteriostatic Water (30mL)
  • 20 Large Needles + Syringes Combo (3cc x 21g x 1)
  • 2 Sterile Empty Glass Vials (10mL)

Kick your research up a notch with one of these comprehensive offerings.

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Bacteriostatic Water vs. Sterile Water

Sterile water is another solvent that is formulated for injections. Its uses overlap with bacteriostatic water, but the two solvents differ in some crucial regards. Let’s weigh the similarities and differences between these two substances.


These are the main similarities between the two [5]:


  • Both are formulated as sterile solvents for injection per USP standards.
  • They both contain sterile water, as bacteriostatic water is simply sterile water with an added preservative.


  • Both are indicated for use to dissolve or dilute compounds for aqueous injection via subcutaneous, intramuscular, and intravenous routes.
  • Common solutes include hormones and peptides.


  • Both are dangerous when injected alone, causing hemolysis.
  • Neither is safe for cerebrospinal fluid injections or use with all medications.


These are the key differences [5]:


  • Bacteriostatic water contains a preservative agent (0.9% benzyl alcohol) to prevent contamination, and sterile water does not.
  • The average pH level of bacteriostatic water is 5.7, while that of sterile water is 5.5.
  • Bacteriostatic water’s alcohol content offers better solubility [6].


  • Sterile water can become contaminated with a single use, while bacteriostatic water remains uncontaminated for as many as 28 days [4, 5].
  • Solutions made with bacteriostatic water may be safely stored to provide multiple doses for up to 28 days. Those made with sterile water must be discarded within four hours of reconstitution [20, 21].


  • Subjects with benzyl alcohol allergies should not be administered a solution reconstituted with bacteriostatic water.
  • Drug interactions may vary.

Overall, bacteriostatic water is a safer and more convenient solvent for use in HGH injections due to its extended shelf life and reduced risk of contamination.

Does Bacteriostatic Water Need to Be Refrigerated?

There is no need to refrigerate pure bacteriostatic water due to its bacteriostat agent. The benzyl alcohol combats contamination for several weeks after opening. Unopened units remain sterile for years if properly stored.

The storage guidelines for bacteriostatic water are as follows [3, 4]:

  • Vials should be kept at 60-80° F. This applies to both sealed and unsealed containers.
  • Cabinets are ideal for storage, as vials should be kept out of direct sunlight.
  • Avoid placing items on top of vials, as this could cause damage.

When mixed with other substances, the storage guidelines will likely change. Refer to product labels in these cases.

Bacteriostatic Water for HGH

Does HGH Need to Be Refrigerated?

Yes, it is recommended to refrigerate HGH powder and reconstituted solutions to prevent degradation. However, some HGH powders may be safe at room temperature before reconstitution.

After reconstitution, the HGH solution is less stable and should be refrigerated in most cases. Read product labels to determine the exact temperature requirements.

The typical storage temperature range for bacteriostatic water reconstitutions is 36-46°F, which prevents contamination for up to four weeks. Keep HGH out of direct sunlight, and do not freeze it, as this will compromise the quality [18].

Bacteriostatic Water and HGH | Overall

Bacteriostatic water is an excellent sterile solvent for use in HGH injections. Not only does it reduce the risk of infection, but it saves researchers the hassle of having to reconstitute HGH powder with every dose. HGH solutions can be safely stored for up to 28 days and yield multiple doses.

To boost your HGH research, purchase your bacteriostatic water kit from Bacteriostaticwater.org, the best online source for bacteriostatic water and complementary materials.

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  2. Bacteriostatic Water for Injection [Internet]. Drugs.com. 2021 [cited 2022Aug17]. Available from: https://www.drugs.com/pro/bacteriostatic-water-for-injection.html
  3. Jackson S. The complete guide on Bacteriostatic Water [Internet]. MedLab International. MedLab International; 2019 [cited 2022Aug17]. Available from: https://medlabgear.com/blogs/articles/guide-bacteriostatic-water
  4. The uses of bacteriostatic water and where to get it [Internet]. Farris Laboratories, Inc. Farris Laboratories, Inc.; 2021 [cited 2022Aug17]. Available from: https://www.farrislabs.com/blogs/information/the-uses-of-bacteriostatic-water-and-where-to-get-it
  5. Bacteriostatic water vs. Sterile Water: The differences that Can save your life [Internet]. Bacteriostaticwater.com. 2020 [cited 2022Aug17]. Available from: https://www.bacteriostaticwater.com/blogs/news/bacteriostatic-water-vs-sterile-water-the-differences-that-can-save-your-life
  6. Deusmedical. Bacteriostatic water vs. Sterile Water  [Internet]. DeusMedical.com. [cited 2022Aug17]. Available from: https://deusmedical.com/articles/which-one-to-choose-bacteriostatic-water-vs-sterile-water
  7. Hawkins M. 3 facts you need to know about bacteriostatic water: Performance forum [Internet]. Performance Forum | Join The Debate. 2019 [cited 2022Aug18]. Available from: https://www.performanceforum.org/3-facts-you-need-to-know-about-bacteriostatic-water/
  8. Feasby TE, Hahn AF, Gilbert JJ. Neurotoxicity of bacteriostatic water. N Engl J Med. 1983 Apr 21;308(16):966-7. doi: 10.1056/NEJM198304213081614. PMID: 6687625.
  9. Bacteriostatic water for injection, USP warnings and precautions [Internet]. bacteriostatic water for injection, USP Warnings and Precautions | Pfizer Medical Information – US. 2022 [cited 2022Aug18]. Available from: https://www.pfizermedicalinformation.com/en-us/node/657396/pi_section/field_spl_warnings
  10. Rapido F. The potential adverse effects of haemolysis. Blood Transfus. 2017 May;15(3):218-221. doi: 10.2450/2017.0311-16. PMID: 28518048; PMCID: PMC5448827.
  11. HGH (human growth hormone): What it is, benefits & side effects [Internet]. Cleveland Clinic. 2021 [cited 2022Aug26]. Available from: https://my.clevelandclinic.org/health/articles/23309-human-growth-hormone-hgh
  12. Genentech: Understanding human growth hormone [Internet]. Genentech: Understanding Human Growth Hormone. 2022 [cited 2022Aug28]. Available from: https://www.gene.com/patients/disease-education/understanding-human-growth-hormone
  13. Bidlingmaier M, Strasburger CJ. Growth hormone. Handb Exp Pharmacol. 2010;(195):187-200. doi: 10.1007/978-3-540-79088-4_8. PMID: 20020365.
  14. Growth hormone injections: Uses and side effects [Internet]. Medical News Today. MediLexicon International; [cited 2022Aug26]. Available from: https://www.medicalnewstoday.com/articles/312905#adults
  15. Dunkin MA. HGH (human growth hormone): Uses and side effects [Internet]. WebMD. WebMD; [cited 2022Aug26]. Available from: https://www.webmd.com/fitness-exercise/human-growth-hormone-hgh
  16. Hintz RL. Growth hormone: uses and abuses. BMJ. 2004 Apr 17;328(7445):907-8. doi: 10.1136/bmj.328.7445.907. PMID: 15087325; PMCID: PMC390151.
  17. Growth hormone, athletic performance, and aging [Internet]. Harvard Health. 2021 [cited 2022Aug27]. Available from: https://www.health.harvard.edu/diseases-and-conditions/growth-hormone-athletic-performance-and-aging
  18. Dimitar Marinov MD. How to inject HGH safely: Best sites for injections: HFS Clinic [Internet]. HFS Clinic [HGH & TRT]. HFS Clinic medical staff; 2022 [cited 2022Aug27]. Available from: https://hghfor-sale.com/blog/how-to-inject-hgh-correctly/
  19. Marinov DD. How and where to inject HGH (for best results): Hrtguru Clinic [Internet]. HRTGuru. 2022 [cited 2022Aug27]. Available from: https://hrtguru.com/how-to-inject-hgh-properly-guide/
  20. Abernathy DS. How to mix and store HGH: Best water for mixing HGH [Internet]. HRTGuru. 2022 [cited 2022Aug24]. Available from: https://hrtguru.com/water-to-mix-hgh/
  21. Sterile water for injection, USP – Food and Drug Administration [Internet]. [cited 2022Aug27]. Available from: https://www.accessdata.fda.gov/drugsatfda_docs/label/2016/018632s051lbl.pdf

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