Titus Thorne

Last Updated November 6, 2022

Titus Thorne

 November 6, 2022

Researchers interested in investigating peptides for skin repair and hair growth may be curious about how to calculate GHK-Cu dosage for their next experiment.

Past studies have shown that GHK-Cu offers numerous positive biological actions including tissue repair, cell protection, and multiple anti-cancer and anti-inflammatory actions. Yet despite significant interest in GHK-Cu, comparatively little is known about how this peptide should be dosed in experimental settings. 

This GHK-Cu dosage calculator and guide will highlight the main benefits of this peptide and illuminate possible avenues for further research. Dosages used in past studies will be discussed in the context of calculating appropriate and effective GHK-Cu doses in future experiments. 

For researchers interested in working with GHK-Cu, our top recommendations for reliable GHK-Cu vendors will be shared at the end of this guide, including one vendor that ships research-grade GHK-Cu worldwide and another that offers peptides like GHK-Cu in cosmetic formulations.

Let’s get to it. 

Buy GHK-Cu from our #1 recommended vendor...

Disclaimer: Peptides.org contains information about products that are intended for laboratory and research use only, unless otherwise explicitly stated. This information, including any referenced scientific or clinical research, is made available for educational purposes only. Peptides.org makes every effort to ensure that any information it shares complies with national and international standards for clinical trial information and is committed to the timely disclosure of the design and results of all interventional clinical studies for innovative treatments publicly available or that may be made available. However, research is not considered conclusive. Peptides.org makes no claims that any products referenced can cure, treat or prevent any conditions, including any conditions referenced on its website or in print materials.


What is GHK-Cu?

GHK-Cu is a peptide (a short chain of amino acids) that is made up of three amino acids; glycine, histidine, and lysine. Together these form GHK, or glycyl-l-histidyl-l-lysine [1].

GHK bonds easily to copper, thereby becoming GHK-Cu. That is why it is also called copper peptide or copper tripeptide. GHK-Cu is produced naturally in our bodies and is found in blood, saliva, and urine.

GHK-Cu seems to play a sizable role in repairing DNA in cells [2]. DNA is essentially a blueprint that cells use when they split and create new cells. When the DNA gets damaged, as is normal over time, that can result in poorer duplication of cells and impaired ability to generate new tissue. GHK-Cu helps repair and reset the DNA, leading to improved tissue growth [2].

When we age, the genes that are involved in inflammation and tissue destruction become more active. The genes that regenerate and repair tissue become less active. GHK-Cu appears to help turn back the clock: it reactivates the regenerative genes and deactivates the inflammatory and destructive ones [2].

GHK-Cu has also been linked to several other processes that support tissue creation and has been found to:

  • Stimulate the synthesis of collagen in skin cells [3]
  • Increase the accumulation of protein [3]
  • Increase synthesis of decorin [4]
  • Stimulate the synthesis of metalloproteinases
  • Suppress the production of the inflammatory cytokine interleukin-6 [2]
  • Stimulate antioxidant gene activity [2]

Essentially, GHK-Cu is believed to help the body break down old tissue, repair DNA, and create new, healthier tissue.


ghk-cu


Benefits of GHK-Cu?

There are numerous potential benefits associated with GHK-Cu administration. Here is a breakdown of potential benefits reported in the literature:

  • Anti-pain action [1]
  • Anti-anxiety effects [1]
  • Anti-aggression effects [1]
  • Antioxidant protection [1]
  • Repairing wounds [2]
  • Lung and gut tissue healing [2]
  • Hair growth [2]
  • Skin repair, wrinkle reduction, and anti-aging effects [5]
  • Tissue remodeling [6]
  • Antibacterial effects and infection reduction [7]
  • Nerve regeneration [8]

Below, we’ll go deeper into the main potential benefits of GHK-Cu.


GHK-Cu for Anti-Aging/Skin

GHK-Cu is probably best known for its supposed anti-aging properties. It has been well-studied as an anti-aging agent and is considered to be an activator of tissue remodeling. In one animal study, GHK-Cu was found to promote cell proliferation and angiogenesis, thereby accelerating scald wound healing. This research has been used to support its inclusion in many cosmetic products [9].

In one study involving women with sun-damaged skin, GHK-Cu treatment removed visible signs of aging after 12 weeks. It improved skin firmness, elasticity, and clarity as well as reduced wrinkles and fine lines [5]. The peptide showed similar results in a separate clinical trial and outperformed products containing vitamin K, vitamin C, and retinoic acid in reducing skin aging [10].

Based on Dr. Loren Pickart’s work, GHK-Cu has been shown to [5]:

  • Tighten skin
  • Improve elasticity and firmness
  • Improve skin density
  • Reduce deep wrinkles and fine lines
  • Reduce hyperpigmentation and sun damage.

ghk-cu


GHK-Cu for Healing

GHK-Cu has potential uses as a healing agent. It has not only been shown to help wounds close faster with less chance of infection, but it also seems to be involved in healing ulcers and regenerating lung tissue [1, 2]. It does so by repairing DNA, accelerating tissue growth, and increasing levels of antioxidant enzymes. Separate research has shown that GHK-Cu peptide helps slow down the growth of cancerous tissue and prevent its formation [4, 11].


GHK-Cu for Hair Growth

A number of studies have suggested that GHK may be beneficial for hair growth. Data from these studies indicates that GHK-Cu may increase hair follicle size and thus improve hair growth and thickness [1, 2, 13].

This research suggests that GHK-Cu may be beneficial in subjects suffering from hair loss or hair thinning, clearly highlighting a possible avenue for further research [13].


GHK-Cu for Muscle Growth

There is no published data from human clinical trials to support the notion that GHK-Cu stimulates muscle growth. However, given that it has been shown to possess antioxidant and anti-inflammatory activity [6], GHK-Cu may decrease recovery time following resistance training. There is a clear need for more research in this area.

Now that we’ve reviewed the main potential benefits of GHK-Cu, let’s now turn our attention to the question at hand: what is the proper way to dose this peptide?


GHK-Cu Dosage Guide | What Researchers MUST Know

Based on the available research, GHK-Cu is considered to be safe and well-tolerated when administered to test subjects [1]. According to Dr. Pickart, credited with discovering GHK-CU, “no issues (involving GHK-Cu) have ever arisen during its use as a skin cosmetic or in human wound healing studies” [2].

That said, researchers may find it useful to have a bit of background on past GHK-Cu dosing protocols.

Minimum effective dose

The lowest known doses of GHK-Cu administered to test subjects involve the application of skin creams and topical ointments. Skin creams containing GHK-Cu are usually applied once or twice a day to affected areas—usually to the face for skin tightening and wrinkles, to the scalp for hair growth, or other areas for scars or wound healing [10]. When administered in this manner, GHK-Cu appears to be non-toxic and non-irritating with no adverse effects [2].

When injected, there is little data to indicate the minimum effective dose of GHK-Cu [11]. Where GHK-Cu is sold as a powder that is reconstituted for injection, a typical starting dose is 1 mg per day over a treatment course.

Effective dose

The effective dose of GHK-CU depends on the route of administration, whether that is via injection, a topical cream, or tablet.

CHK-Cu injections

Subcutaneous injections are a common way to administer GHK-Cu to test subjects for the purpose of studying systemic healing, skin rejuvenation, and hair growth.

Researchers and anti-aging doctors typically administer 1-2 mg/daily of GHK-Cu over the course of a 30-day treatment.

Skin creams and skin patches

GHK-Cu skin creams and patches seem to be able to pass therapeutically effective amounts of this copper peptide through the skin and into the body [14].

  • 0.5 micrograms/kg seems to be effective as a daily dose for lowering anxiety. That would be 35 μgs for a 70kg person [15].
  • 50 mg seems to be effective as a person’s daily dose for systemic healing [11].

Of course, topicals may vary in their formulas and exact GHK-Cu content, so it is always prudent to read the instructions for the product before administering it to test subjects. 

Liposomal encapsulated oral tablets

There is no evidence that administering GHK-Cu in regular pill form produces any effects, as it is broken down in the intestines. However, research indicates that liposomal encapsulated tablets may be effective when dosed at 10 mg per day or more [11].

Lethal dose

GHK can lower blood pressure, so a large enough dose may have serious consequences. However, the lethal dose of GHK-Cu peptide is very high – around 21,000 mg for a 70kg human (or about 330 mg/kg) [13].

Recommended dose

As a research peptide, there is no “recommended dose” for GHK-Cu. However, a review of the available literature suggests that researchers have previously dosed it in the following ways [2]:

  • GHK-Cu Dose: 1-2 mg per day, injected subcutaneously or intramuscularly.
  • Frequency: Once per day. You can also split the dose into several smaller injections.
  • Duration of Cycle: 30 days.

We do not encourage the unsupervised self-administration of GHK-Cu or any other research peptide—under any circumstances.


Where to Buy GHK-Cu Online? | 2022 Guide

For researchers looking for injectable GHK-Cu, Peptide Sciences is the best place to buy GHK-Cu online. They offer research-grade peptides, which means researchers will get pure peptides with no fillers. Besides carrying the best quality peptides on the net, they offer:

  • Easy, secure, and convenient payment options. They accept all major credit cards, and they also accept cryptocurrencies like BTC and ETH.
  • Convenient shipping. They ship anywhere in the world. Researchers based in the United States can expect delivery in as little as three days. Orders over $100 qualify for free shipping, while those over $400 include 30ml of bacteriostatic water.
  • Great customer service. Peptide Sciences is friendly and helpful. If researchers have any issues, they can get in touch with an actual person who will work hard to resolve the issue.

We’ve had only great experiences with Peptide Sciences and highly recommend them.

For researchers looking for a GHK-Cu skin cream, our go-to is Aseir Custom. Their products, including their GHK-Cu Sky Blue Cream for anti-aging, are all manufactured to the highest standards. They also ship anywhere in the world and offer a great refund policy.

Buy GHK-Cu from our #1 recommended vendor...


How to Order GHK-Cu | Country-by-Country

Both Peptide Science and Aseir Custom accept orders from all over the world. To order GHK-Cu injections from any country, simply visit their website and place an order.

Researchers based internationally should receive their shipment within 7-10 business days.


ghk-cu


Bacteriostatic Water for Injection

To correctly handle peptides such asGHK-Cu, researchers must use a standard set of tools, including sterile needles, bacteriostatic water, and alcohol wipes.

The important task of gathering all these materials may be a challenge for some researchers. Trying to source the full set of items from multiple sites can be time-consuming and cut into research activities.

If you find yourself struggling to get equipped, don’t worry! Peptides.org is glad to name our most highly recommended provider of all the materials needed to properly carry out peptide research.

BacteriostaticWater.org

This online retailer tops our list of suppliers with its impressive selection of high-grade research kits, fitted with the requisite tools for peptide reconstitution, administration, and storage. Rather than wasting hours shopping for products from multiple sources, order a complete research kit from BacteriostaticWater.org. Choose from two convenient options.

The deluxe starter research kit is a great choice to jumpstart your experimentation, complete with:

  • Bacteriostatic Water (30mL) – 3x
  • Insulin Syringes (0.5 cc/mL x 29g x ½) – 100x
  • Alcohol Prep Pads – 200x
  • Sterile Empty Glass Vial (10mL) – 1x
  • Large Needles + Syringes Combo (3cc x 21g x 1) – 10x

More seasoned analysts may prefer the premium research kit, which boasts:

  • Bacteriostatic Water (30mL) – 5x
  • Insulin Syringes (0.5 cc/mL x 29g x ½) – 200x
  • Alcohol Prep Pads – 200x
  • Sterile Empty Glass Vial (10mL) – 2x
  • Large Needles + Syringes Combo (3cc x 21g x 1) – 20x

Enjoy your research with a top-caliber toolkit from our favorite approved supplier.

Buy Bacteriostatic Water from our #1 recommended vendor...


GHK-Cu Dosage | Verdict

Hopefully, this guide has answered the questions of researchers interested in calculating the optimum GHK-Cu dosage for their next experiment.  

Based on our review of the available research, doses of 1-2 mg per day of reconstituted injectable GHK-Cu offer numerous therapeutic benefits in test subjects including anti-inflammatory action, tissue repair, hair growth, and more.

Researchers looking to find research-grade GHK-Cu for their next study may consider visiting our recommended vendor for injectable peptides: Peptide Sciences, or our recommended vendor for GHK-Cu cream, Aseir Custom instead.


References

  1. Pickart L, Margolina A. Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data. Int J Mol Sci. 2018 Jul 7;19(7):1987. doi: 10.3390/ijms19071987. PMID: 29986520; PMCID: PMC6073405.
  2. Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2014). GHK and DNA: resetting the human genome to health. BioMed Research International, 151479. https://doi.org/10.1155/2014/151479
  3. Maquart, F. X., Pickart, L., Laurent, M., Gillery, P., Monboisse, J. C., & Borel, J. P. (1988). Stimulation of collagen synthesis in fibroblast cultures by the tripeptide‐copper complex glycyl‐L‐histidyl‐L‐lysine‐Cu2+. FEBS letters, 238(2), 343-346. doi:10.1016/0014-5793(88)80509-x
  4. Siméon, A., Wegrowski, Y., Bontemps, Y., & Maquart, F. X. (2000). Expression of glycosaminoglycans and small proteoglycans in wounds: modulation by the tripeptide–copper complex glycyl-l-histidyl-l-lysine-Cu2+. Journal of Investigative Dermatology, 115(6), 962-968.
  5. Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2015). GHK peptide as a natural modulator of multiple cellular pathways in skin regeneration. BioMed Research International, 648108. https://doi.org/10.1155/2015/648108
  6. Pickart, L. (2008). The human tri-peptide GHK and tissue remodeling. Journal of Biomaterials Science, Polymer Edition, 19(8), 969-988.
  7. Mulder, G. D., Patt, L. M., Sanders, L., Rosenstock, J., Altman, M. I., Hanley, M. E., & Duncan, G. W. (1994). Enhanced healing of ulcers in patients with diabetes by topical treatment with glycyl‐l‐histidyl‐l‐lysine copper. Wound Repair and Regeneration, 2(4), 259-269.
  8. Ahmed, M. R., Basha, S. H., Gopinath, D., Muthusamy, R., & Jayakumar, R. (2005). Initial upregulation of growth factors and inflammatory mediators during nerve regeneration in the presence of cell adhesive peptide‐incorporated collagen tubes. Journal of the Peripheral Nervous System, 10(1), 17-30.
  9. Wang X, Liu B, Xu Q, Sun H, Shi M, Wang D, Guo M, Yu J, Zhao C, Feng B. GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis. Wound Repair Regen. 2017 Apr;25(2):270-278. doi: 10.1111/wrr.12520. Epub 2017 Apr 27. PMID: 28370978.
  10. Abdulghani, A. A., Sherr, A., Shirin, S., Solodkina, G., Tapia, E. M., Wolf, B., & Gottlieb, A. B. (1998). Effects of topical creams containing vitamin C, a copper-binding peptide cream and melatonin compared with tretinoin on the ultrastructure of normal skin-A pilot clinical, histologic, and ultrastructural study. Disease Management and Clinical Outcomes, 4(1), 136-141.
  11. Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2017). The effect of the human peptide GHK on gene expression relevant to nervous system function and cognitive decline. Brain Sciences, 7(2), 20.
  12. Uno, H., & Kurata, S. (1993). Chemical agents and peptides affect hair growth. Journal of Investigative Dermatology, 101(1), S143-S147. https://doi.org/10.1016/0022-202X(93)90516-K
  13. Pickart, L., Vasquez-Soltero, J. M., & Margolina, A. (2012). The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging: implications for cognitive health. Oxidative medicine and cellular longevity, 2012. Doi: 10.1155/2012/324832
  14. Hostynek, J. J., Dreher, F., & Maibach, H. I. (2010). Human skin retention and penetration of a copper tripeptide in vitro as function of skin layer towards anti-inflammatory therapy. Inflammation Research, 59(11), 983-988.
  15. Bobyntsev, I. I., Chernysheva, O. I., Dolgintsev, M. E., Smakhtin, M., & Belykh, A. E. (2015). Effect of Gly-His-Lys peptide and its analogs on pain sensitivity in mice.

Table of Contents
    Add a header to begin generating the table of contents