Last Updated February 9, 2024

 February 9, 2024

Looking for information about fragment 176-191 dosage? 

As the lipolytic fragment of the larger hGH protein, fragment 176-191 was isolated to offer benefits like: 

  • Fat loss and weight management 
  • Improved body fat distribution 
  • Cartilage regeneration 

In this guide, we explain the potential uses and side effects of this popular compound and include a fragment 176-191 dosage calculator for interested researchers. 

At the end, we reveal our go-to supplier of frag 176-191 and other research peptides for fat loss.

Buy Fragment 176-191 from our top-rated vendor...

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Fragment 176-191 Dosage Chart | Quick Breakdown

Timeframe Daily Fragment 176-191 Dosage
Weeks #1-4 200-500mcg (initiate at lowest dose to assess tolerance)
Week #5 Washout
Weeks #6-9 200-500mcg
Weeks #10 Washout

  • Treatment Protocol: administered once or twice daily (on an empty stomach) subcutaneously

What is Fragment 176-191?

Fragment 176-191 refers to the peptide fragment of human growth hormone (hGH) comprising HGH amino acids 176 to 191. This section of hGH is known as its C-terminus and is believed to be responsible for the main fat-reducing benefits of hGH [1].

Clinical trials have shown that Fragment 176-191 administration can reduce fat mass and overall body weight [1]. The peptide is additionally modified via the attachment of tyrosine and the N-terminus, which improves its stability. Thus, it is also known as Tyr-hGH 177-191 or alternative as Anti-Obesity Drug 9604 (AOD9604) [1].

The fat loss and metabolic benefits of intact hGH have been widely researched and are well understood [2, 3]. However, severe side effects associated with long-term hGH treatment, such as glucose intolerance and hypertension, have made long-term research using intact hGH unviable.

Based on the literature, Fragment 176-191 has a favorable tolerance and safety profile compared to intact hGH. In the following section, we’ll look more closely at the potential benefits offered by this research peptide.

fragment 176-191 dosage

Fragment 176-191 Benefits and Uses

Based on data from clinical trials, HGH Fragment 176-191 appears to offer a number of benefits. In this section, we’ll outline the main potential benefits and uses of this peptide according to the available evidence.

Potential alternative to intact hGH

All published evidence points toward HGH Fragment 176-191 having a good safety profile in laboratory animals and human study volunteers. Its use is not associated with hGH side effects like insulin resistance, and it appears to be a safer alternative for researchers who want to investigate the fat-reducing properties of hGH without using intact hGH and exposing test subjects to its risks.

The carboxy-terminus of hGH, known as hGH 177-191, appears to have a strong impact in terms of mobilizing lipids in the body—a key benefit of hGH [1]. On the other hand, there is no evidence that the Fragment 176-191 presents some of the hGH-associated risks like edema, hypertension, acromegaly, diabetes, or glucose intolerance. In fact, data from clinical trials shows that this peptide produces adverse events at a rate indistinguishable from placebos [1].

Strong effect on lipid mobilization

The second potential benefit of HGH Fragment 176-191 is its strong impact on lipid mobilization. Studies on intact hGH have shown that it helps inhibit lipoprotein lipase activity in adipose tissue [4] and promotes lipolysis in adipocytes. Other studies have shown a positive impact on fat mass cells [5], fat mass [6], and overall fat distribution [7]. Research involving HGH Fragment 176-191 has shown that it temporarily increases blood glucose and causes a sustained rise in plasma insulin levels [8].

A 2001 study invovling obese mice and beta(3)-AR knock-out mice found that Fragment 176-191 increased thermogenic activity in the rodents’ skeletal muscle [9]. This finding suggests that Fragment 176-191’s potential uses could include treating prediabetes, Type 2 diabetes, and obesity in humans [1].

Enhanced cartilage regeneration

A 2013 study found that when white rabbits suffering from osteoarthritis (OA) were injected with Fragment 176-191 in conjunction with hydrochloric acid (HA), they experienced enhanced cartilage growth compared with those receiving HA injections alone [10].

While these results have yet to be replicated in human clinical trials, the study’s authors suggested that as Fragment 176-191 does not stimulate the production of IGF-1, it “may be safer than human recombinant GH for the long-term treatment of OA” [10].

In the following section, we will review the potential side effects of HGH Fragment 176-191.

Fragment 176-191 Side Effects

At the time of writing, a total of six clinical trials involving HGH Fragment 176-191 have been conducted. Data from these trials shows that this peptide has a positive safety profile and does not produce a high rate of adverse events (AEs) [1].

The six trials involved fewer than 900 participants and all were given regular physical examinations including vital sign tests, ECG tests, blood glucose tests, and IGF-1 tests. Two trials involved single intravenous injections of Fragment 176-191 dosed between 25mcg – 400mcg/kg body weight, while four trials involved orally administered (tablets or pills) Fragment 176-191 dosed between 1mg – 54mg.

Researchers found that subjects receiving Fragment 176-191 experienced no increases in IGF-1 levels, and no negative impact on carbohydrate metabolism, glucose tolerance, or insulin resistance [1]. Further, the rate and type of AEs experienced by the volunteers were indistinguishable from those experienced by the group receiving the placebo.

Apart from the lack of the more severe effects typically associated with hGH use, minor side effects from Fragment 176-191 injections could include irritation or pain at the site of injection.

In the following section, we’ll take a look at how HGH Fragment 176-191 was dosed in these trials and explore how researchers may dose this peptide in their own studies.

Fragment 176-191 Dosage Calculator and Guide

For researchers interested in how best to dose HGH Fragment 176-191, the below dosage calculator and guide should help calculate an appropriate dosage for study participants.

How to Administer HGH Fragment?

HGH Fragment 176-191 typically comes in dry powder form and must be reconstituted with bacteriostatic water before being injected subcutaneously or intramuscularly.

However, in two of the six clinical trials conducted to date, HGH Fragment 176-191 was administered intravenously, and in the remaining four trials it was administered orally via capsules or tablets.

What Dose of Fragment 176-191 Should Be Administered?

Researchers should note that clinical studies on Fragment 176-191 to date have not involved administration via subcutaneous injection, but have employed infusion instead, as four escalating single doses of 25mcg – 400mcg/kg, with a washout week between each administration [1].

However, the subcutaneous route of administration is often preferred for administration of research peptides.

Due to a lack of clinical data, researchers aiming to investigate the weight loss potential of subcutaneous Fragment 176-191 are recommended to employ more conservative doses.

A common HGH Fragment 176-191 dose for fat loss research based on information from HRT clinics ranges from 200-500mcg daily, subcutaneously.

When Should HGH Fragment Be Administered?

HGH Fragment 176-191 is commonly administered on an empty stomach, 1-2 times daily.

If administered once, it should be done in the AM, at least one hour before breakfast. If it’s administered twice per day, the second dose should come in the PM, a couple of hours after that day’s final meal.

How Often Should Fragment 176-191 Be Administered?

Based on data from clinical trials involving HGH Fragment 176-191, doses can be safely administered daily—either in a single or two injections, one in the AM and the second in the PM [1].

More frequent administration may be superior due to the peptide’s short half-life, which is reported to be about 3 min following intravenous infusion [11].

However, as Fragment 176-191 is a research chemical and in vivo testing is prohibited, there remains a paucity of published data on administration via subcutaneous injection.

How Long Should Fragment 176-191 Be Administered?

Based on data from clinical trials with oral administration, Fragment 176-191 can be administered daily for up to 24 weeks [1].

However, due to the lack of data on subcutaneous administration, researchers are advised to begin with shorter studies, spanning no longer than a month.

A prudent Fragment 176-191 cycle length would be 4 weeks followed by a washout period of at least one week.

fragment 176-191 dosage

Where to Buy Fragment 176-191 Online? | 2024 Edition

HGH Fragment 176-191 is freely available as a research chemical to qualified scientists.

In our experience, however, the quality of this peptide can vary from one vendor to the next.

The team has extensively reviewed the top peptide sources, concluding that the following vendor currently offers the best value for money along with the highest quality peptides.


PureRawz is the best option for researchers looking for research-grade HGH fragment 176-191.

Here’s why:

  • Third-Party Testing: PureRawz gets each peptide batch tested by a third-party lab before listing it for sale.
  • Secure Checkout: PureRawz website uses the latest SSL technology to ensure that no data or personal information are exposed. Researchers’ confidentiality is highly valued and protected at all times.
  • Superb Service Team: PureRawz service team is second to none. They offer rapid email support so researchers can rest assured that any question or issue they may have will be swiftly resolved.

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How to Reconstitute Fragment 176-191

As mentioned, Fragment 176-191 is available for research as lyophilized powder that must be reconstituted via a suitable solvent such as bacteriostatic water.

During the process of reconstitution, researchers will also need other essential materials, such as sterile vials, alcohol wipes, and needles.

Having the proper supplies on hand is a key part of safe and effective research. For some researchers, it may be a challenge to acquire all the essential supplies, as not all retailers carry the required items. However, it is required to have certain materials on hand to reconstitute research peptides.

These materials include:

  • Alcohol Prep Pads
  • Sterile Empty Glass Vials
  • Large Needles + Syringes
  • Bacteriostatic Water
  • Insulin Syringes

Fragment 176-191 Dosage | The Verdict?

For researchers curious about HGH Fragment 176-191 dosage, we trust that this guide has provided a sufficient overview of how this peptide may be dosed in research settings. HGH Fragment 176-191 clearly offers avenues for research in areas such as weight management without causing typical hGH-related side effects.

Fat loss research on Fragment 176-191 commonly involves subcutaneous administration of the peptide at 200-500mcg daily. The total dose may be delivered once in the AM, or it may spread across two doses—one in the AM and another in the PM, in both cases on an empty stomach.

Research involving Fragment 176-191 is usually conducted over the course of four weeks, before cycling off.

If Fragment 176-191 could be the right peptide for the research study, be sure to check out our top-rated supplier for the highest-purity research chemicals at the best possible prices.


  1. Stier, Heike et al. “Safety And Tolerability Of The Hexadecapeptide AOD9604 In Humans”. Jofem.Org, 2021,
  2. Ho KK, O'Sullivan AJ, Hoffman DM. Metabolic actions of growth hormone in man. Endocr J. 1996 Oct;43 Suppl:S57-63. doi: 10.1507/endocrj.43.suppl_s57. PMID: 9076343.
  3. Vijayakumar A, Novosyadlyy R, Wu Y, Yakar S, LeRoith D. Biological effects of growth hormone on carbohydrate and lipid metabolism. Growth Horm IGF Res. 2010;20(1):1-7.
  4. Gravhølt CH, Schmitz O, Simonsen L, Bülow J, Christiansen JS, Møller N. Effects of a physiological GH pulse on interstitial glycerol in abdominal and femoral adipose tissue. Am J Physiol. 1999 Nov;277(5):E848-54. doi: 10.1152/ajpendo.1999.277.5.E848. PMID: 10567011.
  5. Rodríguez-Arnao J, Jabbar A, Fulcher K, Besser GM, Ross RJ. Effects of growth hormone replacement on physical performance and body composition in GH deficient adults. Clin Endocrinol (Oxf). 1999 Jul;51(1):53-60. doi: 10.1046/j.1365-2265.1999.00737.x. PMID: 10468965.
  6. Takahashi S, Satozawa N. The 20-kD human growth hormone reduces body fat by increasing lipolysis and decreasing lipoprotein lipase activity. Horm Res. 2002;58(4):157-64. doi: 10.1159/000065488. PMID: 12324712.
  7. Rosenbaum M, Gertner JM, Leibel RL. Effects of systemic growth hormone (GH) administration on regional adipose tissue distribution and metabolism in GH-deficient children. J Clin Endocrinol Metab. 1989 Dec;69(6):1274-81. doi: 10.1210/jcem-69-6-1274. PMID: 2685009.
  8. Ng FM, Bornstein J. Hyperglycemic action of synthetic C-terminal fragments of human growth hormone. Am J Physiol. 1978 May;234(5):E521-6. doi: 10.1152/ajpendo.1978.234.5.E521. PMID: 645904.
  9. Heffernan M, Summers RJ, Thorburn A, Ogru E, Gianello R, Jiang WJ, Ng FM. The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice and beta(3)-AR knock-out mice. Endocrinology. 2001 Dec;142(12):5182-9. doi: 10.1210/endo.142.12.8522. PMID: 11713213.
  10. Kwon DR, Park GY. Effect of Intra-articular Injection of AOD9604 with or without Hyaluronic Acid in Rabbit Osteoarthritis Model. Ann Clin Lab Sci. 2015 Summer;45(4):426-32. PMID: 26275694.
  11. Moré MI, Kenley D. Safety and metabolism of AOD9604, a novel nutraceutical ingredient for improved metabolic health. Journal of Endocrinology and Metabolism. 2014 Jun 27;4(3):64-77.

Scientifically Fact Checked by:

David Warmflash, M.D.

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