Titus Thorne

Last Updated March 13, 2023

Titus Thorne

 March 13, 2023

Curious about Bacteriostatic Water for injections?

Then you're in luck! This guide has you covered.

Bacteriostatic water is in many cases the best solvent or dilutant for injections of medications and other therapeutic substances, such as peptides and hormones. Its preservative content keeps it sterile to prevent infection, and its chemical properties allow it to efficiently dissolve solutes without compromising their integrity.

This guide will explore key information on the use of bacteriostatic water for injections. You will learn its various applications, how it differs from sterile water, and your best online source to buy bacteriostatic water kits.

If you are a peptide researcher or laboratory professional, you can safely enjoy the benefits of bacteriostatic water with proper use. 

Buy Bacteriostatic Water from our #1 recommended vendor...

Disclaimer: Peptides.org contains information about products that are intended for laboratory and research use only, unless otherwise explicitly stated. This information, including any referenced scientific or clinical research, is made available for educational purposes only. Peptides.org makes every effort to ensure that any information it shares complies with national and international standards for clinical trial information and is committed to the timely disclosure of the design and results of all interventional clinical studies for innovative treatments publicly available or that may be made available. However, research is not considered conclusive. Peptides.org makes no claims that any products referenced can cure, treat or prevent any conditions, including any conditions referenced on its website or in print materials.

What is Bacteriostatic Water?

Composed of sterile water and 0.9% benzyl alcohol and an organic preservative agent, bacteriostatic water resists bacterial growth and contamination [1]. It is commonly used to reconstitute or dilute therapeutic substances for injection, including intramuscular, intravenous, intradermal, and subcutaneous routes [1, 2].

Bacteriostatic water formulations for injection have pH levels of 4.5-7, averaging 5.7. These formulations are less expensive to produce and more widely available than comparable products, such as sterile water [3].

A mainstay in medical facilities, research labs, and beyond, bacteriostatic water resists contamination for up to 28 days after initial use. Unused vials can remain viable for years with proper storage [4]. It is considered an ideal sterile solution due to its low toxicity and minimal interference with drug effectiveness.

However, bacteriostatic water for injection may not be the best option in all cases, such as in neonatal or pregnant recipients, or in cases of subjects being administered certain medications [3, 5].

When preparing bacteriostatic water for injection, all product indications should be closely followed, including recommended dosages and injection schedules [4].

bacteriostatic water for injections

What is Bacteriostatic Water Used For?

The primary uses of bacteriostatic water are as follows:

  1. It is used as a solvent or dilutant for drugs or therapeutic compounds that call for aqueous injection.
  2. The preservative agent (0.9% benzyl alcohol) in bacteriostatic water allows packaging in multi-dose vials that resist contamination for up to 28 days when used with sterile needles. This significantly reduces the risk of infection [4].
  3. It is an efficient solvent and presents minimal interaction with most medications [6].
  4. Apart from medications, bacteriostatic water is also indicated for the reconstitution and injection of therapeutic research peptides and hormones [7].
  5. Suitable injection routes are determined by the type of solute, the recipient’s needs, and target rates of diffusion and metabolization. These include subcutaneous, intramuscular, intravenous, and intradermal injection routes [1].

When using bacteriostatic water for injections, certain precautions must be followed. Handlers should adhere to the exact indications or medical instructions. Contraindications for bacteriostatic water injections include newborn babies and cerebrospinal injections [2, 8].

Additionally, bacteriostatic water must always be mixed with a solute before injection. Failure to do so risks the development of hemolysis, damaging the red blood cells [9, 10].

Adverse reactions are rare and can include fever, infection, and thrombosis. If symptoms appear, injections should be discontinued pending medical consultation [9].

Bacteriostatic Water for Injections

Bacteriostatic water is commonly used for the injection of therapeutic compounds. Now we will explore some of the most exciting applications.

Bacteriostatic Water for Peptides

Research peptides present great potential in emerging clinical contexts, such as combating symptoms of cardiovascular and metabolic diseases, as well as improving reproductive health [11]. Typically available as lyophilized powders to maintain purity and integrity, peptides require reconstitution before injection [12].

Bacteriostatic water works well as a peptide solvent, reconstituting the compounds without compromising their effectiveness. Plus, peptide solutions made with bacteriostatic water resist contamination. Experts state that it may be necessary to run trials in order to select the best solvent for various peptides, and sodium chloride water should be avoided [12].

This is the method of peptide reconstitution with bacteriostatic water for injection [13]:

  1. Gather the following: alcohol swabs, bacteriostatic water, peptide powder, and a sterile syringe.
  2. Wipe down all materials with an alcohol swab.
  3. Withdraw approximately 1mL of bacteriostatic water from the vial and slowly transfer it into the peptide container.
  4. Do not agitate the solution, which can damage the peptides. Allow the powder to naturally dissolve until the vial is transparent. If available, sonication may be performed in order to speed up the process.
  5. If you notice that the powder does not properly dissolve, try a stronger solvent.
  6. Be sure to consult indications for exact dosage and concentration.

Bacteriostatic Water for HGH (Human Growth Hormone)

Human growth hormone has a range of therapeutic applications, from enhanced physical composition and performance, to treatment of hormone deficiency in adolescents and children [14, 15].

Injection of HGH is considered safe when prescribed by a doctor. Exogenous formulations are available in powder and liquid forms. For the reconstitution of HGH powder, bacteriostatic water is a great choice, as it preserves HGH solutions and efficiently dissolves lyophilized aggregates [16].

The proper reconstitution of HGH before injection closely follows the above method. Injections are best suited to fatty areas, such as the thigh or waist. Handlers should follow the applicable dosage and administration schedule [16]. For safe storage, adhere to manufacturer indications.

Bacteriostatic Water for HCG (Human Chorionic Gonadotropin)

The clinical applications of the hormone hCG include fertility enhancement in both women and men, as well as a supplement to TRT in male subjects [17, 18].

Often available as a lyophilized powder, hCG is best reconstituted with bacteriostatic water for injection due to the solvent’s efficiency and preservative agents. HCG solutions are to be refrigerated to prevent degradation [19].

Reconstitution follows the above method. The dosage and administration schedule should follow applicable recommendations [18, 19].

bacteriostatic water for injections

Where to Buy Bacteriostatic Water Online | 2023 Guide

If you are interested in using bacteriostatic water for injections, look no further!

Your best option for assembling the necessary tool kit is a single, reputable source. Don’t waste time and energy attempting to source syringes, alcohol swabs, and bacteriostatic water from multiple retailers. Not only is this inefficient, but you may come across low-quality products from inferior manufacturers.

To make it easy for you to get the best materials, the Peptides.org team has perused the web and selected the single best online source for your injection material requirements:


This trusted vendor has an impressive selection of full research kits for your convenience.

The starter set has all you need to get going, including the following:

  • 200 Alcohol Prep Pads
  • 100 Insulin Syringes (0.5 cc/mL x 29g x ½)
  • 3 vials of Bacteriostatic Water (30mL)
  • 10 Large Needles + Syringes Combo (3cc x 21g x 1)
  • 1 Sterile Empty Glass Vial (10mL)

If you want a little more, check out the premium kit:

  • 200 Alcohol Prep Pads
  • 200 Insulin Syringes (0.5 cc/mL x 29g x ½)
  • 5 vials of Bacteriostatic Water (30mL)
  • 20 Large Needles + Syringes Combo (3cc x 21g x 1)
  • 2 Sterile Empty Glass Vials (10mL)

Qualified handlers are sure to enjoy this top online source for research materials. With a deluxe kit, you will have all you need for safe injections at your disposal. Don’t wait to enjoy the benefits of bacteriostatic water for injections.

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Bacteriostatic Water vs. Sterile Water

Both substances are sterile solvents formulated for use in medical settings. Though their features overlap, bacteriostatic water and sterile water have several significant differences. Let’s explore their points of similarity and difference to understand their appropriate uses. You may be surprised to learn that they are not interchangeable [5].

First, let’s break down the basics of sterile water. This solution has no antimicrobial agents. It is simply water that has gone through purification and distillation processes for its safe use in various therapeutic contexts, namely injections, dilution, and irrigation. Because it is vulnerable to contamination, it is sold in single-dose containers [5].


These are the primary similarities between bacteriostatic water and sterile water [5]:

  1. Both sterile solutions are formulated for medical use.
  2. Both act as dilutants or solvents of substances to enable injection.
  3. Both may be used in the injection of peptides, hormones, and various medications, often reconstituted from lyophilized powder.
  4. Both are suitable for subcutaneous intravenous, intramuscular, and intradermal injections.
  5. Both have the dangerous side effect of hemolysis when injected alone.
  6. Both are unsafe for use in neonates.
  7. Both meet US Pharmacopeia (USP) standards.


Here are the key differences between bacteriostatic water and sterile water [5]:

  1. Their pH levels and chemistry are distinct. Bacteriostatic water has a 0.9% benzyl alcohol content and an average pH level of 5.7, while sterile water is free of additives with a pH level of 5.0-7.0.
  2. Bacteriostatic water’s preservative content prevents contamination for multiple doses, while sterile water may be contaminated after one use. So, the former is available in multi-dose containers that are viable for up to 28 days after opening, while sterile water comes in single-dose vials. The re-use of sterile water for injections is strictly prohibited.
  3. Bacteriostatic water solutions remain sterile and safe for longer due to the preservative agent.
  4. FDA indications vary regarding drug interactions. Some drugs can interact poorly with each solvent, and they are not considered universally interchangeable in clinical contexts.
  5. Sterile water is formulated for irrigation, such as rinsing wounds, unlike bacteriostatic water.
  6. Bacteriostatic water should not be used on individuals with benzyl alcohol allergies.
  7. Bacteriostatic water has wider availability and is simpler to manufacture.
  8. Specialists generally consider bacteriostatic water to be a more efficient solvent or dilutant.

Despite their similarities, bacteriostatic water and sterile water differ in a few key areas. Overall, specialists agree that bacteriostatic water for injection is safer and more effective in most cases. Its preservative content reduces the risk of contamination and infection, boosts shelf life, and enables optimal solvency for aqueous injection [6].

There may be cases in which sterile water is more appropriate, as outlined above. Follow medical advice and relevant indications for maximum safety and results when using bacteriostatic water for injections.

Bacteriostatic Water for Injections | Overall

In closing, bacteriostatic water is often the preferred choice for injections in a range of contexts. Made with the organic preservative benzyl alcohol, this solution resists contamination to enable multiple doses per vial. Plus, it is a great dilutant for a range of drugs and therapeutic substances, facilitating aqueous delivery without compromising potency or effectiveness.

Purchase your full kit today to safely use bacteriostatic water for injections. From medications to peptides and hormones, bacteriostatic water is a winning option when it comes to reconstitution and sterility. Complement your research with this USP solvent, the go-to solution of the medical and scientific community.

Buy Bacteriostatic Water from our #1 recommended vendor...


  1. W. B. What is Bacteriostatic Water? [Internet]. Study.com | Take Online Courses. Earn College Credit. Research Schools, Degrees & Careers. 2022 [cited 2022Aug17]. Available from: https://study.com/academy/lesson/what-is-bacteriostatic-water-definition-uses.html
  2. Bacteriostatic Water for Injection [Internet]. Drugs.com. 2021 [cited 2022Aug17]. Available from: https://www.drugs.com/pro/bacteriostatic-water-for-injection.html
  3. Jackson S. The complete guide on Bacteriostatic Water [Internet]. MedLab International. MedLab International; 2019 [cited 2022Aug17]. Available from: https://medlabgear.com/blogs/articles/guide-bacteriostatic-water
  4. The uses of bacteriostatic water and where to get it [Internet]. Farris Laboratories, Inc. Farris Laboratories, Inc.; 2021 [cited 2022Aug17]. Available from: https://www.farrislabs.com/blogs/information/the-uses-of-bacteriostatic-water-and-where-to-get-it
  5. Bacteriostatic water vs. Sterile Water: The differences that Can save your life [Internet]. Bacteriostaticwater.com. 2020 [cited 2022Aug17]. Available from: https://www.bacteriostaticwater.com/blogs/news/bacteriostatic-water-vs-sterile-water-the-differences-that-can-save-your-life Deusmedical. Bacteriostatic water vs. Sterile Water  [Internet].
  6. DeusMedical.com. [cited 2022Aug17]. Available from: https://deusmedical.com/articles/which-one-to-choose-bacteriostatic-water-vs-sterile-water
  7. Hawkins M. 3 facts you need to know about bacteriostatic water: Performance forum [Internet]. Performance Forum | Join The Debate. 2019 [cited 2022Aug18]. Available from: https://www.performanceforum.org/3-facts-you-need-to-know-about-bacteriostatic-water/
  8. Feasby TE, Hahn AF, Gilbert JJ. Neurotoxicity of bacteriostatic water. N Engl J Med. 1983 Apr 21;308(16):966-7. doi: 10.1056/NEJM198304213081614. PMID: 6687625.
  9. Bacteriostatic water for injection, USP warnings and precautions [Internet]. bacteriostatic water for injection, USP Warnings and Precautions | Pfizer Medical Information – US. 2022 [cited 2022Aug18]. Available from: https://www.pfizermedicalinformation.com/en-us/node/657396/pi_section/field_spl_warnings
  10. Rapido F. The potential adverse effects of haemolysis. Blood Transfus. 2017 May;15(3):218-221. doi: 10.2450/2017.0311-16. PMID: 28518048; PMCID: PMC5448827.
  11. Lau JL, Dunn MK. Therapeutic peptides: Historical perspectives, current development trends, and future directions. Bioorg Med Chem. 2018 Jun 1;26(10):2700-2707. doi: 10.1016/j.bmc.2017.06.052. Epub 2017 Jul 1. PMID: 28720325. Peptide Information. Peptide reconstitution [Internet].
  12. Peptide Information. 2019 [cited 2022Aug18]. Available from: https://www.peptidesciences.com/information/peptide-reconstitution/
  13. Rathi KK. How to reconstitute peptides: Complete guide 2022 [Internet]. Muscle and Brawn. 2022 [cited 2022Aug18]. Available from: https://muscleandbrawn.com/peptides/how-to-reconstitute-peptide/
  14. Crist DM, Peake GT, Egan PA, Waters DL. Body composition response to exogenous GH during training in highly conditioned adults. J Appl Physiol (1985). 1988 Aug;65(2):579-84. doi: 10.1152/jappl.1988.65.2.579. PMID: 3170408.
  15. Grumbach MM, Bin-Abbas BS, Kaplan SL. The growth hormone cascade: progress and long-term results of growth hormone treatment in growth hormone deficiency. Horm Res. 1998;49 Suppl 2:41-57. doi: 10.1159/000053087. PMID: 9730672.
  16. Marinov D. 5 steps on how to mix HGH with Bacteriostatic Water [Internet]. Hormone Center -. 2022 [cited 2022Aug18]. Available from: https://hrtmedical.net/a-step-by-step-guide-on-how-to-mix-hgh/
  17. Human chorionic gonadotropin, HCG injection [Internet]. Cleveland Clinic. 2022 [cited 2022Aug18]. Available from: https://my.clevelandclinic.org/health/drugs/18559-human-chorionic-gonadotropin-hcg-injection
  18. Kim ED, Crosnoe L, Bar-Chama N, Khera M, Lipshultz LI. The treatment of hypogonadism in men of reproductive age. Fertil Steril. 2013 Mar 1;99(3):718-24. doi: 10.1016/j.fertnstert.2012.10.052. Epub 2012 Dec 7. PMID: 23219010.
  19. Tressler K. How to mix HCG (human gonadotropin) [Internet]. Defy Medical. 2022 [cited 2022Aug18]. Available from: https://www.defymedical.com/blog/how-to-mix-hcg-human-gonadotropin/

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