Last Updated January 18, 2024

 January 18, 2024

Sleep researchers are increasingly seeking insights and protocols to ensure a proper DSIP dosage for their studies.

Standardized dosing and administration guidelines are necessary for reliable and accurate experiments with DSIP peptide, still an experimental drug.

DSIP is researched for its recognized therapeutic potential, which include observed benefits like:

  • Increasing sleep quality
  • Reducing anxiety
  • Stress reduction

These effects make DSIP an important compound in sleep and psychiatric studies.

In this article, researchers can explore the available data on DSIP dosage and get acquainted with a comprehensive DSIP dosage calculator. This may help researchers improve the effectiveness of their experiments and dig deeper into exploring how DSIP works.

Let’s get started with a concise breakdown of how DSIP may be dosed in a research setting.

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DSIP Dosage Chart | Quick Breakdown

Time frame Daily DSIP dosage for sleep research
Week 1-4 100-250mcg
Week 5-8 Break
Week 9-12 100-250mcg
Week 13-16 Break

What is Delta Sleep-Inducing Peptide?

Delta Sleep-Inducing Peptide (DSIP) is a neuropeptide composed of nine amino acids. It is primarily produced in the central nervous system, and was first isolated from the brains of rabbits that were subjected to sleep by electric stimulation of the intralaminal thalamic area.
Due to its sleep-related synthesis, the peptide has attracted attention for its potential role in regulating sleep patterns and promoting sleep-related well-being [1].

As a molecule that occurs in the brain, DSIP is thought to potentially interact with receptors that modulate the sleep cycle. More specifically, it may interact with N-methyl-D-aspartate (NMDA) receptors, gamma-aminobutyric acid (GABA) receptors, and alpha 1-adrenergic receptors in the central nervous system [1, 2].

Ultimately, this results in the modulation of the sleep’s architecture and optimization of the various stages of sleep, especially the highly restorative slow-wave sleep (SWS), often referred to as deep sleep or stage 3 sleep [1, 2].

Additionally, its involvement in managing stress and reducing anxiety could potentially contribute to enhancing sleep quality. Further, scientists have explored DSIP applications relating to its abilities to [3]:

  • influence hormones
  • protect against stress
  • reduce pain
  • improve withdrawal management

While more research is needed to understand the mechanisms and potential benefits of DSIP, it has piqued the interest of researchers looking to effect better sleep quality and lower stress.

DSIP Dosage Calculator

Benefits of DSIP

Here are some of the most commonly documented benefits of DSIP based on the available literature.

Researchers should note that more investigation is needed to fully comprehend this peptide’s mechanisms and effects.

DSIP for Sleep Improvement

By regulating the sleep-wake cycle, DSIP is thought to help induce sleep, potentially leading to improved sleep quality and rejuvenation [4].

Here are some of the most notable trials and experiments outlining the peptide’s potential:

  • According to Kastsiuchenka et al. (2019), administering DSIP to rodents showed improvement in non-rapid eye movement (NREM) sleep, particularly in deep slow-wave sleep (SWS). The study suggested that DSIP may regulate sleep architecture, leading to better sleep quality and overall well-being [5].
  • Schneider-Helmert et al. (1981) investigated the effects of intermediate-term administration of DSIP on sleep and daytime performance in 14 individuals with insomnia. The study followed a placebo-controlled design, where either DSIP or placebo was administered for seven consecutive nights. Each volunteer in the treatment group received injections of DSIP at a dosage of 25 nanomoles per kilogram. Sleep quality was assessed via polysomnograms, and the results indicated a significant improvement in sleep quality among the participants receiving DSIP [6].

The latter study also suggested that DSIP may also have beneficial effects on stage-4 sleep, which is also known as REM sleep when dreaming occurs. REM sleep may play an important function in processing and consolidating emotional memory [6].

DSIP for Stress Reduction

Some researchers consider that DSIP may help to alter the body's response to stress and enhance sleep quality.

For example, one experiment in rabbits suggested that DSIP may help reduce electrical instability of the heart in emotional stress and mitigate stress-related events such as extrasystoles [7].

Yet, more research is needed to evaluate the potential of DSIP in stress reduction and mood enhancement.

DSIP for Neuroprotection and Withdrawal Management

DSIP has also been suggested to potentially interact with the opioid receptors, which may not only mediate the peptide’s effects on sleep onset and architecture but also have potential benefits in subjects with addiction.

Here are two notable trials underlying these potential benefits:

  • In a study involving 107 patients with alcohol (n = 47) or opiate (n = 60) withdrawal symptoms, DSIP was administered intravenously based on the hypothesis of its agonistic activity on opiate receptors. The study found that DSIP was effective in rapidly improving or completely alleviating withdrawal symptoms in 97% of opiate addicts and 87% of alcoholics. Subjects also experienced significant improvements in levels of anxiety [8].
  • DSIP was evaluated for treating acute opioid withdrawal symptoms in a 21-year-old woman with a history of opioid dependence. The peptide was administered intravenously in varying intervals over seven days. Each administration led to a rapid and marked improvement in withdrawal symptoms, with effects lasting around 3.5 hours [9].

DSIP for Pain Management

DSIP has also been explored for its potential benefits against pain based on its interactions with opioid systems and effects on various biochemical and physiological parameters.

  • A pilot study investigated DSIP's effectiveness in 7 patients suffering from chronic pain, including migraine episodes, vasomotor headaches, chronic tinnitus, and psychogenic pain attacks. The treatment involved intravenous DSIP administration on five consecutive days, followed by five injections every 48-72 hours. The results showed a significant reduction in pain levels for 6 out of the 7 patients. Additionally, there was a notable decrease in associated depressive states [10].
  • DSIP was tested for its antinociceptive effects in mice and rats. When administered into the brain circulation and cerebrospinal fluid of mice, DSIP showed a significant, dose-dependent reduction in pain response in the tail-pinch and hot-plate tests. The antinociceptive impact of DSIP was negated by the opioid antagonist naloxone and was not observed in morphine-tolerant mice, suggesting that DSIP acts at the supraspinal level, possibly influencing opioid receptors [11].

Side Effects of DSIP

DSIP is not approved for human use, and extensive clinical trials are lacking as of writing. Therefore, the side effects and safety of this peptide have not yet been fully explored.

Several small clinical trials have nonetheless reported on the safety and tolerance of DSIP. The largest trial involving DSIP to date, which included a total of 107 volunteers, reported that the peptide was generally well-tolerated with mostly minor side effects, which included [8]:

  • Low blood pressure
  • Increased sweating
  • Headache
  • Nausea
  • Vertigo

Considering that the drop in blood pressure was reported as moderate to severe in some patients, researchers should consider hypotension as a potentially exclusionary criterion for DSIP research.

Smaller trials in volunteers with insomnia also report a favorable safety profile and no side effects during or after DSIP administration [6].

Preclinical studies likewise suggest a favorable safety profile [12, 13]. Nevertheless, the peptide is not cleared for human use, but is currently available to qualified researchers as a reference material for in vitro experimentation.

DSIP Dosage Calculator

Based on the available scientific data, DSIP has been clinically tested in doses of 25nmol/kg of body weight, which, considering the molar mass of DSIP, is equal to about 21.4mcg/kg. This equals about 1500mcg for a 150lb subject.

Researchers have safely applied these doses for up to two weeks based on the available case reports [6, 8, 14].

Anecdotal reports have also stated a dosage range of 100 to 250mcg for DSIP. However, it's important to note that this dosage lacks data-backed evidence.

Researchers are always recommended to start with a low dose within the indicated range and gradually increase as needed while carefully monitoring for any potential side effects. Adjustments to the dosage may be required based on the subjects’ responses and study objectives.

Here is a summary of the most commonly used DSIP dosing protocol based on available reports:

  • Starting DSIP dose: 100mcg administered at night, preferably three hours before bedtime.
  • Frequency: The frequency of administration is typically once daily, according to the available data.
  • Duration: Studies have lasted up to 14 days.
  • Notes: Researchers have not tested doses higher than 21.4mcg/kg/daily.

While the provided dosage ranges may serve as guidelines for in vitro studies, it is important to note that future research findings, may lead to adjustments in these recommendations.

Therefore, it is crucial for researchers to stay up-to-date on the literature to ensure accurate and safe administration of DSIP in their studies.

Microdosing DSIP?

Microdosing refers to administering chemicals at levels below therapeutic doses. The aim of this practice is to minimize adverse effects while exploring potential benefits.

By microdosing DSIP, researchers could potentially observe some of the benefits of the peptide related to sleep, stress, or withdrawal management with minimal to no risk.

However, it's important to note that there are no microdosing studies involving DSIP, and the topic should be approached with caution.

DSIP Cycle Length?

Establishing the proper duration for a DSIP cycle depends primarily on the goals of the research project. Typically, DSIP cycles range from days to a couple of weeks; however, there is no standardized duration currently in place within the scientific community.

When determining the length of a peptide cycle, researchers must also take into account individual responses and potential risks. Furthermore, various factors can influence DSIP cycles, such as product availability, budget limitations, and research protocols.

How to Reconstitute DSIP

Delta sleep-inducing peptide (DSIP) is typically offered in lyophilized powder form, which necessitates meticulous reconstitution for research application.

To enhance the peptide's longevity, a solvent with a non-toxic preservative, such as bacteriostatic water containing 0.9% benzyl alcohol, is advised. This approach helps maintain DSIP's stability and efficacy for up to four weeks when stored in refrigeration between 36 to 46°F (2 to 8°C).

Researchers must note that the reconstitution process also requires several other specific supplies, including:

  • Sterile syringes and needles
  • Alcohol swabs for sterilization
  • A safe disposal container for sharp instruments

The procedure for reconstituting DSIP involves the following steps:

  1. Ensure that the DSIP and bacteriostatic water vials reach room temperature, avoiding exposure to direct light or heat.
  2. Disinfect the tops of both vials with alcohol swabs.
  3. Draw 1ml of air into the syringe, injecting it into the bacteriostatic water vial, and then withdraw the necessary quantity of solvent.
  4. Gently incorporate the solvent into the DSIP vial, with the needle pointed against the vial's wall. Post-use, the needle and syringe should be disposed of safely.
  5. If available, sonication is preferred for dissolution; otherwise, gently roll or swirl the vial until the contents are clear. Vigorous shaking or tapping can adversely affect the peptide's integrity.
  6. The solution should be clear before usage. Any cloudiness signifies contamination, necessitating the solution’s disposal.

For dosage and storage specifics, as well as handling instructions, researchers should refer to the literature and any product labeling.

DSIP Dosage Calculator

Where to Buy DSIP Online? | 2024 Edition

While research-grade DSIP is available for online purchase, buyers should take the time to evaluate potential vendors on factors like peptide quality, transparency, cost, shipping times, and customer service.

At, we’re pleased to recommend a trusted vendor of high-quality research peptides including DSIP.

Limitless Life

Here is why Limitless Life stands out from other vendors we have tried:

  • High Purity DSIP with HPLC-MS Testing: Limitless Life guarantees that their peptide products all meet minimum purity standards, which they verify through third-party high-performance liquid chromatography and mass spectrometry (HPLC-MS) testing.
  • Convenient Website: The Limitless Life website makes ordering peptides a hassle-free process. Researchers can easily navigate the site, access detailed product descriptions, and choose forms and quantities of DSIP for their investigations.
  • Excellent Customer Care: Limitless Life prides itself on its customer service. Their knowledgeable staff is available to address any inquiries regarding their products by phone or email.

In sum, researchers can rely on Limitless Life for quality, convenience, security, and excellent customer support.

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Delta Sleep-Inducing Peptide Dosing Guide | Verdict

DSIP holds a significant place in the realm of sleep research. Yet the effective implementation of DSIP in research settings hinges on adhering to optimal dosing strategies.

While definitive dosing guidelines for DSIP are yet to be established, researchers can rely on prevailing dosing recommendations as a preliminary starting point.

DSIP is typically administered subcutaneously, with doses varying between 100-250mcg. The dosing frequency can range from daily to weekly, depending on the subject’s individual response and maintenance requirements.

Researchers must also remember to initiate DSIP research at the lowest effective dose and adjust it incrementally, staying vigilant for any adverse reactions.

Visit our top recommended provider to obtain DSIP and other research peptides to ensure successful experimentation.


  1. Graf MV, Schoenenberger GA. Delta sleep-inducing peptide modulates the stimulation of rat pineal N-acetyltransferase activity by involving the alpha 1-adrenergic receptor. J Neurochem. 1987 Apr;48(4):1252-7. doi: 10.1111/j.1471-4159.1987.tb05654.x. PMID: 3029331.
  2. Tukhovskaya EA, Ismailova AM, Shaykhutdinova ER, Slashcheva GA, Prudchenko IA, Mikhaleva II, Khokhlova ON, Murashev AN, Ivanov VT. Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke. Molecules. 2021 Aug 26;26(17):5173. doi: 10.3390/molecules26175173. PMID: 34500605; PMCID: PMC8434407.
  3. Graf, M. V., & Kastin, A. J. (1986). Delta-sleep-inducing peptide (DSIP): an update. Peptides, 7(6), 1165–1187.
  4. Schneider-Helmert D. (1984). DSIP in insomnia. European neurology, 23(5), 358–363.
  5. K Nakagaki, S Ebihara, S Usui, Y Honda, Y Takahashi. Sleep-promoting effect following intracerebroventricular injection of a phosphorylated analogue of delta sleep-inducing peptide (DSIP-P) in rats. Neuroscience Letter1988 Aug 31;91(2):160-4.
  6. D Schneider-Helmert, G A Schoenenberger.The influence of synthetic DSIP (delta-sleep-inducing-peptide) on disturbed human sleep. Experientia 1981;37(9):913-7
  7. Arkhangel'skaia MI, Zviagintseva MA. Vliianie peptida del'ta-sna na élektricheskuiu nestabil'nost' serdtsa pri émotsional'nom stresse [Effect of delta-sleep inducing peptide on electrical instability of the heart in emotional stress]. Patol Fiziol Eksp Ter. 1989 Mar-Apr;(2):45-9. Russian. PMID: 2755725.
  8. Dick P, Costa C, Fayolle K, Grandjean ME, Khoshbeen A, Tissot R. DSIP in the treatment of withdrawal syndromes from alcohol and opiates. Eur Neurol. 1984;23(5):364-71. doi: 10.1159/000115715. PMID: 6548969.
  9. Soyka M, Rothenhaeusler HB. Delta sleep-inducing peptide in opioid detoxification. Am J Psychiatry. 1997 May;154(5):714-5. doi: 10.1176/ajp.154.5.714b. PMID: 9137140.
  10. Larbig W, Gerber WD, Kluck M, Schoenenberger GA. Therapeutic effects of delta-sleep-inducing peptide (DSIP) in patients with chronic, pronounced pain episodes. A clinical pilot study. Eur Neurol. 1984;23(5):372-85. doi: 10.1159/000115716. PMID: 6548970.
  11. Nakamura A, Nakashima M, Sugao T, Kanemoto H, Fukumura Y, Shiomi H. Potent antinociceptive effect of centrally administered delta-sleep-inducing peptide (DSIP). Eur J Pharmacol. 1988 Oct 18;155(3):247-53. doi: 10.1016/0014-2999(88)90510-9. PMID: 2853064.
  12. Graf MV, Kastin AJ, Coy DH, Zadina JE.DSIP reduces amphetamine-induced hyperthermia in mice. Physiol Behav. 1984 Aug;33(2):291-5.
  13. Tsunashima K, Masui A, Kato N. The effect of delta sleep-inducing peptide (DSIP) and phosphorylated DSIP (P-DSIP) on the apomorphine-induced hypothermia in rats.84;23(5):364-71.
  14. Schneider-Helmert D. Effects of DSIP on narcolepsy. Eur Neurol. 1984;23(5):353-7. doi: 10.1159/000115713. PMID: 6548968.

Scientifically Fact Checked by:

Dimitar Marinov, Ph.D.

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